Coding

Part:BBa_K3001006:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-14)


Cas13a Lsh Coding Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1828
    Illegal PstI site found at 2197
    Illegal PstI site found at 2926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1828
    Illegal PstI site found at 2197
    Illegal PstI site found at 2926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 572
    Illegal BglII site found at 1196
    Illegal BglII site found at 1592
    Illegal BglII site found at 1883
    Illegal BglII site found at 1973
    Illegal BglII site found at 3134
    Illegal BglII site found at 3221
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1828
    Illegal PstI site found at 2197
    Illegal PstI site found at 2926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1828
    Illegal PstI site found at 2197
    Illegal PstI site found at 2926
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 558
    Illegal SapI.rc site found at 664
    Illegal SapI.rc site found at 1534
    Illegal SapI.rc site found at 2368


Design Notes

Needs to be overexpressed in an E. coli strain that contains rare codons.


Source

Ordered from Addgene.

References